Cis-acting Elements Stimulating Kinetoplastid Guide RNA-directed Editing

Guide to the nomenclature of kinetoplastid RNA editing: a proposal.

In vitro uridine insertion RNA editing mediated by cis-acting guide RNAs.

Uridine (U) insertion/deletion editing of mitochondrial mRNAs in kinetoplastid protozoa is a posttranscriptional process mediated by guide RNAs (gRNAs). The gRNAs direct the precise insertion and deletion of Us by a cleavage-ligation mechanism involving base pairing. We show that a cognate gRNA in cis at the 3' end of a preedited NADH dehydrogenase 7 (ND7) mRNA substrate can direct U insertions...

The Impact of mRNA Structure on Guide RNA Targeting in Kinetoplastid RNA Editing

Mitochondrial mRNA editing in Trypanosoma brucei requires the specific interaction of a guide RNA with its cognate mRNA. Hundreds of gRNAs are involved in the editing process, each needing to target their specific editing domain within the target message. We hypothesized that the structure surrounding the mRNA target may be a limiting factor and involved in the regulation process. In this study...

cis Recognition elements in plant mitochondrion RNA editing.

RNA editing in higher plant mitochondria modifies mRNA sequences by means of C-to-U conversions at highly specific sites. To determine the cis elements involved in recognition of an editing site in plant mitochondria, deletion and site-directed mutation constructs containing the cognate cox II mitochondrial gene were introduced into purified mitochondria by electroporation. The RNA editing stat...

Cis–Acting RNA Elements of Human Immunodeficiency Virus

The World Health Organization has indicated that effective control of the HIV/AIDS pandemic is the world’s most urgent public health challenge. The 2009 UNAIDS Global Facts and Figures report estimated that almost 60 million people have been infected with the virus and that 25 million AIDS-related deaths have occurred since the pandemic began in the early 1980s. In 2008, there were approximatel...